Figure 3
From: Ubp43 gene expression is required for normal Isg15 expression and fetal development
Fetal chorioallantoic placental concentrations of Isg15. Panel A represents a western blot of placental free Isg15 (~17-kDa) and conjugated Isg15 (> 30-kDa) on 17.5 dpc. Panel B represents quantitation of free placental Isg15. Panel C represents quantitation of conjugated placental Isg15. Null fetal-derived placental tissue had greater concentrations of Isg15 when compared to pooled wt/het placenta. An interaction (P < 0.05) of genotype by dpc suggested that null fetal-derived placental tissue had greater concentrations of Isg15 conjugates on 17.5 dpc in contrast to other genotypes or null tissue on 12.5 dpc. Means are LSM ± SE. * denotes P < 0.05.