Figure 2
From: Ubp43 gene expression is required for normal Isg15 expression and fetal development
Northern blot analysis for Ubp43 and Isg15 (panel A). Fetal chorioallantoic placental RNA was analyzed for Ubp43 and Isg15 using cDNA probes. Isg15 mRNA was present on 12.5 and 17.5 dpc in all genotypes. Ubp43 mRNA was present in wt and het tissues at the correct size. 18S rRNA was not different. Panel B represents a graphical interpretation of the expression of Isg15 in fetal chorioallantoic placenta. Isg15 mRNA was greater in null derived placenta in comparison to wt or het placenta. Means are LSM ± SE and represent 3 +/+, 3 +/-, and 2 -/- on 12.5 dpc, and 3 +/+, 2 +/- and 2 -/- fetal chorioallantoic placenta. * denotes P < 0.07.