Figure 1

Illustration of fetal development and Ubp43 genotype on day 17.5 of pregnancy (A) and description of how implantation sites were collected (B). A litter from a day 17.5 pregnant mouse shows that all Ubp43 null offspring were dead. Genotype ratio was 2:2:1 (wt:het:null) on day 17.5. On day 12.5, 75% of Ubp43 null mice were dead with a genotype ratio of 2:5:1. There also was a significant loss of +/- fetuses between 12.5 and 17.5 suggesting that Ubp43 gene dosage may also be involved. Panel B shows a cross-sectional representation of a mouse uterus illustrating the various tissues collected for protein and RNA analysis. Anti-mesometrial (AM) represents uterine tissue surrounding the fetal compartment. Fetal chorioallantoic placenta (CP) represents fetal-derived placental tissue. Mesometrial decidua (MM) represents uterine tissue in direct contact with fetal-derived placenta.