Figure 5

mRNA for androgen receptors at the ventral prostate. The level of mRNA for androgen receptors at the ventral prostate was modulated by copulation. The graph shows that mRNA was detected in males before sexual behavior (white bar). The level of mRNA increased constantly during the first three ejaculations, reaching the highest value after the third ejaculation. However, the concentration returned to baseline levels after four ejaculations. RT-PCR gels at the top show bands of mRNA for β-actin (β-Ac mRNA) and the androgen receptor (mRNA AR); bp = base pair; 1–4 = number of ejaculations. * = p < 0.05; ** = p < 0.01.