Figure 4
PGF2α has no effect on BKCa channel activity. A Representative recordings from the same membrane patch (+40mV) in the cell-attached configuration after addition of 20 μM NS1619 and subsequent addition of 1 μM PGF2α. PGF2α did not reverse NS1619-stimulated channel activity. B Each bar represents the average channel open probability obtained from cell attached patches (n = 4; +40 mV) before and 15 min after each addition (n = 4). *Significant increase in channel activity compared to control. C & D THG113.31 activates the BKCa channel independently of the FP receptor. Representative recordings from the same membrane patch (+40mV) in the cell-attached configuration before and after addition of 1 μM PGF2α and subsequent addition of 10 μM THG113.31. Each bar represents the average channel open probability obtained from cell attached patches (n = 3; +40 mV) before and 15 min after each addition (n = 3). *Significant increase in channel activity compared to control.