Figure 3
THG113.31 opens BKCa channel activity in human myometrial smooth muscle cells in a concentration-dependent manner. A Activity plot of BKCa channel open probability in a single cell-attached patch before and 15 min after exposure to cumulative doses of 10 and 50 μM THG113.31. Vertical bars are channel activity during a 100-ms test pulse to +40 mV. Recording time under each condition was 10 s, as indicated on the time axis. Breaks in the time axis represent drug incubation periods when channel activity was not recorded. Period of drug exposure is indicated by horizontal lines above the histogram. B Each bar represents the average channel open probability obtained from cell attached patches (+40 mV) before and 15 min after additions of 10 and 50 μM THG113.31, respectively. *Significant increase in channel activity compared to control (n = 6; P < 0.005)