Figure 8

Quantitative RT-PCR for Nanog , Oct-4 and Sox-2 expression in embryonic disc, PUC cells and fibroblasts. Known concentrations of total RNA from day 14–15 embryonic discs (Disc, black line), early passage PUC cells (PUC, green line) and porcine fibroblasts (Fibro, red line) were subjected to quantitative RT-PCR using gene specific primers for Nanog, Oct-4, Sox-2 and 18S ribosomal subunit. 200 ng template RNA from each reaction was used here. The cycle number is shown on the X-axis and log₂ fluorescence is shown on the Y-axis. Horizontal line in each graph indicates the threshold.