Multiple sequence alignment of rat DEFB protein sequences. Conserved 6-cysteine array is shown in red. Consensus posttranslational modification sites are indicated: double underlined – N-glycosylation; single underlined – casein kinase II phosphorylation; dotted underlined – protein kinase C phosphorylation. ^ indicates predicted signal peptide cleavage site. Sequence shown in bold was cloned to test the antibacterial activity.