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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: ICI 182,780 has agonistic effects and synergizes with estradiol-17 beta in fish liver, but not in testis

Figure 2

Gene expression in E 2 -treated (high dose) sea bream. Sea bream males received coconut oil implants with 10 mg/kg body weight E2 (E), 10 mg/kg ICI (I) or coconut oil alone (CTL) for 28 h. Liver (A and C) and testis (B and D) were analyzed for gene expression of estrogen receptors (ERα, ERβa, ERβb) and estrogen-responsive genes, vitellogenin (VgII) and choriogenin (ChgL) by semi-quantitative RT-PCR. Each bar (A and B) is the mean ± S.E.M. of the relative expression values (target gene/18S) of eight fish. Different letters indicate statistically significant differences between treatments (general linear model using log10 of the relative expression values, P < 0.05). The gel images (C and D) are representative RT-PCR products for each experimental group.

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