Figure 3
Effect of noradrenaline (NA) in the celiac ganglion and LH in the ovary on the production of ovarian androstenedione (A2), using the celiac ganglion-SON-ovary system obtained from rats on day 15 of pregnancy. The systems were incubated in Krebs-Ringer solution, at 37°C in an atmosphere of 95% O2-5% CO2 for 180 min. LH (50 ng/ml) was added to the ovarian compartment and ascorbic acid (0.1 mg/ml in Krebs-Ringer) without (Control-LH group) and with NA (10-6 M) added to the ganglion compartment (NA-LH group). Control group was in absence of NA in the ganglion compartment and LH in the ovarian compartment. In the NA group, 10-6 M NA was added to the ganglionic compartment. Results are expressed as mean ± S.E.M. of six animals per group. Repeated measures analysis of variance followed by Tukey's test was used. The same font denote differences of statistical significance; ap < 0.05 between 60 and 180 min of Control-LH group; b,c and d p < 0.05 in Control-LH group compared with Control group; ep < 0.01 NA-LH group compared with NA group.; *p < 0.01 and • p < 0.05 in NA-LH group compared with Control-LH group; • p < 0.05 in NA group compared with Control group.