Figure 4

Time-course of marker gene expression during differentiation of bovine theca cells in culture. Long-term cultures of bovine theca cells containing minimal amounts of insulin (100 ng/ml) were set up with (filled circles) or without (open circles) the addition of 10 ng/ml bovine luteinizing hormone (LH). At the time-points indicated, CYP11A1, STAR, and INSL3 transcripts were quantified by real time-PCR as described in Materials and Methods. The results of one representative out of three experiments are shown. Data are expressed as mean ± standard deviation. Significant differences (P < 0.05) to the unstimulated cultures at day 8 are indicated by asterisks.