Transactivation domains of NR5A1 wildtype and mutants over-expressed in bovine theca cells. In the ΔAF-2 mutant the essential core region of the AF-2 activating function has been deleted. In the AF-1 mutant amino acid serine 203 has been substituted by alanine preventing the MAP kinase-dependent phosphorylation of this site (A). Northern blot analysis of wild-type and mutant mRNAs transcribed from NR5A1-expressing recombinant adenoviruses in infected HeLa cells (Control: AdEasy adenoviral vector without insert). As a control for equal RNA loading a probe specific for human ribosomal protein RPS27A was used (B). Western blot analysis of NR5A1 protein from adenovirus-infected HeLa cells and rabbit reticulocyte lysate in vitro translation using an antiserum directed against bovine NR5A1 (Control: AdEasy adenoviral vector without insert) (C).