Figure 2
From: Effects of in vitro fertilization and embryo culture on TRP53 and Bax expression in B6 mouse embryos
Expression of Trp53 mRNA within preimplantation stage embryos. RTPCR was performed with specific primers to detect β-Actin (positive control) or Trp53. The negative control (No RT) – Trp53 primers but no reverse transcriptase. 1. liver mRNA positive control, 2. oocytes. 3. zygotes. 4. early 2-cell stage. 5. late 2-cell stage, 6. morulae, and 7. blastocyst. Each analysis was performed on groups of 20 embryos and results are representative of 4 replicates.