Hormonal regulation of mPRβ and PGMRC1 mRNA abundance in full-grown intact rainbow trout ovarian follicles. The experiment was repeated 3 times and for each experiment, all follicles originated from the same ovary. For each hormonal treatment (C = Control; 17,20βP = 17,20β-dihydroxy-4pregnen-3one, 40 ng/ml; G23, G188 = partially purified gonadotropins, 23 or 188 ng/ml; E2 = estradiol, 1 μg/ml) the 20 h incubation was performed in triplicates. The mRNA abundance (mean ± SEM, N = 3 groups of 25 follicles) of each gene was determined by real-time PCR and normalized to the abundance of 18 S. For each experiment, the mRNA abundance of each gene was arbitrarily set to 1 for the control group. *: significantly different from the control group of the experiment at p < 0.05. For each hormonal treatment, occurrence of germinal vesicle breakdown (GVBD) observed after 60 h is indicated below the graphs.