VEGF immunostaining in rats with increased ovarian NGF as a consequence of SON dissection. The predominant location of VEGF was the inner layer of the granulosa compartment, as depicted by arrows. A and B show the staining of control and denervated ovaries, respectively. Negative control is shown in C. Mean ± SEM for H-Score from n = 3, are shown in D. * p < 0.05 vs control. Original magnification 40×. Bar = 150 μm.