TGFβ1 immunostaining in neonatal rat ovaries cultured for 24 hours with NGF. Arrows depict the predominant location of TGFβ1 at the oocytes. A and B show the staining of control and NGF-treated ovaries, respectively. A negative immunohistochemistry is shown in C. Mean ± SEM for H-Score from n = 5 are shown in D. Original magnification 100×. Bar = 50 μm.