Figure 3From: Effects of nerve growth factor (NGF) on blood vessels area and expression of the angiogenic factors VEGF and TGFbeta1 in the rat ovaryVEGF immunostaining in neonatal rat ovaries cultured for 24 hours with NGF. Arrows depict the predominant location of VEGF in granulosa cells of the ovarian follicles (A-B). Arrowheads indicate the presence of VEGF in other cell types of the tissue (C-D), such as oocytes. A and C show the staining of representative control ovaries, and B and D the respective NGF-treated tissues. A negative immunohistochemistry is shown in E. Mean ± SEM for densitometry from n = 5, are shown in F. * p < 0.05 vs control. Original magnification 100Ă—. Bar = 50 μm.Back to article page