VEGF immunostaining in neonatal rat ovaries cultured for 24 hours with NGF. Arrows depict the predominant location of VEGF in granulosa cells of the ovarian follicles (A-B). Arrowheads indicate the presence of VEGF in other cell types of the tissue (C-D), such as oocytes. A and C show the staining of representative control ovaries, and B and D the respective NGF-treated tissues. A negative immunohistochemistry is shown in E. Mean ± SEM for densitometry from n = 5, are shown in F. * p < 0.05 vs control. Original magnification 100×. Bar = 50 μm.