Figure 1From: Effects of nerve growth factor (NGF) on blood vessels area and expression of the angiogenic factors VEGF and TGFbeta1 in the rat ovaryPCR products for the cDNAs evaluated in this study. Pannels A, B and C show photographs of agarose electrophoresis for representative PCR products of the different target sequences studied: Pannel A shows PCR products for VEGF (102 bp for VEGF 120 and 234 bp for VEGF 164) of control samples (C) and NGF treated ovaries (N); additional bands corresponding to other VEGF isoforms were also visible for some samples and thus could not be considered in this study. Pannel B shows PCR product for TGFβ1 (246 bp) of control (C) and NGF treatment (N); Pannel C shows PCR products for the constitutive gene Cyclophilin (350 bp) of control (C) and NGF treatment (N). Negative controls in each photograph correspond to the lane indicated as (-). Pannels D, E and F represent graphs of PCR reactions starting with different quantities of cDNA, indicating the linear range in which each densitometry was made in order to observe differences in each experimental condition: D) Linear range for VEGF amplification (r2 = 0.9936 for VEGF 120 and r2 = 0.9863 for VEGF 164); E) Linear range for TGFβ1 amplification (r2 = 0.9996); F) Linear range for Cyclophilin amplification (r2 = 0.9988). Each graph represents PCR products obtained from a pool of cDNA samples.Back to article page