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Table 2 Primers used for the sense and antisense real time PCR (qRT-PCR) of IAP and MuERV-L.

From: Transcriptional and post-transcriptional regulation of retrotransposons IAP and MuERV-L affect pluripotency of mice ES cells

Transposon

Primer 5'-3'

Tm (°C)

IAP-RTF1

TCAAGGACAGGGTATTGTTG

For reverse transcription

IAP-RTR2

TATTGACGCCCTGGACATCAC

For reverse transcription

IAP-PCRF3

GGGTATTGTTGAGCGTGCGC

56

IAP-PCRR4

TCGGGTGAGTCTTTCTGGTAC

56

MuERV-RTF1

CAAAGTGGCCATGGTGGTCG

For reverse transcription

MuERV-RTR2

GTACCATATATCGAGCGCTG

For reverse transcription

MuERV-PCRF3

TGCTTGGGCTCAGCAACATGG

56

MuERV-PCRR4

GACAGAATGCCTCATCTATCGT

56

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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