Table 1 Primers used for the RT-PCR of genes commonly expressed in differentiated and undifferentiated ES cells.
Gene | Primer 5'-3' (Forward/Reverse) | Size | UniGene |
|---|---|---|---|
GATA-4 | GCCTGTATGTAATGCCTGCG/CCGAGCAGGAATTTGAAGAGG | 469 | Mm. 247669 |
GATA-2 | ACCCACGCCACCCAAAGAAGTG/GCCGCCTTCCATCTTCATGCTC | 157 | Mm. 272747 |
AFP | TTTTCTGAGGGATGAAACCTATG/AAGCTCTTGTTTCATGGTCTGTA | 116 | Mm. 358570 |
Msx-1 | GCTATGACTTCTTTGCCACTCG/TTAAGAGAAGGGGACCAGGTGG | 1016 | Mn. 259122 |
Brachyury | GCTGTGACTGCCTACCAGCAGAATG/GAGAGAGAGCGAGCCTCCAAAC | 220 | Mn. 913 |
Myf5 | TGCCATCCGCTACATTGAGAG/CCGGGGTAGCAGGCTGTGAGTTG | 352 | Mn. 4984 |
Keratin-15 | CACCACATTCTTGCAAAC/ATTAAGGTTCTGCATGGTC | 313 | Mn. 38498 |
HNF3-β | GGACGTAAAGGAAGGGACTCCAC/AGCCCATTTGAATAATCAGCTCAC | 174 | Mn. 938 |
Nestin | AGTGTGAAGGCAAAGATAGC/TCTGTCAGGATTGGGATGGG | 316 | Mn. 23742 |
Vimentin | AAGGGTGAGTAGAGAGTTC/AACACTGTTAGGAAAGAGG | 222 | Mn. 7 |
β3-tubulin | TCACTGTGCCTGAACTTACC/GGAACATAGCCGTAAACTGC | 318 | Mn. 40068 |
Nanog | AGGGTCTGCTACTGAGATGCTCTG/CAACCACTGGTTTTTCTGCCACCG | 363 | Mn. 6047 |
Oct3/4 | GGAGAGGTGAAACCGTCCCTAGG/AGAGGAGGTTCCCTCTGAGTTGC | 391 | Mn. 17031 |
Rex1 | CCAGGGAAGGATGAGAGA/TAGAAGCTGGTAACAGGGAG | 264 | Mn. 3396 |
GENESIS | TCTTACATCGCGCTCATCAC/TCTTGACGAAGCAGTCGTTG | 171 | Mn. 4758 |
FGFR-4 | TCCGACAAGGATTTGGCAG/GCACTTCCGAGACTCCAGATAC | 400 | Mn. 4912 |
TERF1 | TTCAACAACCGAACAAGTGTC/TCTCTTTCTCTTCCCCCTCC | 215 | Mn. 4306 |
Cx43 | TACCACGCCACCACTGGCCCA/ATTCTGGTTGTCGTCGGGGAAATC | 290 | Mn. 4504 |
GLUT1 | CAGTCAGCAATGAAGTCCAG/AGCAGTAAGTTCTCAGCCTC | 585 | Mn. 30044 |
BCRP1/ABCG1 | CCATAGCCACAGGCCAAAGT/GGGCCACATGATTCTTCCAC | 326 | Mn.196728 |
GAPDH | GGGTGTGAACCACGAGAAATATGA/CCTTCCACAATGCCAAAGT | 250 | Mm.379644 |