Induction of rat p120 gene expression in the ovary. Real-time PCR was performed using each specific primer of rat p120, ER-beta, or PR, respectively. Total RNA was isolated from cultured granulosa cells treated with FSH for 48 hr (a) or with FSH for 48 h followed by hCG (b). Each value represents the mean ± SEM of 4 independent Real-time PCR experiments. Different letters for the same graph indicate statistically significant differences (P < 0.05; Duncan multiple comparison test).