Real-time PCR experiment 3. Representative real-time PCR experiments for expression of PRAB (upper panel) and PRB (bottom panel) mRNAs in uterus from rats subjected to treatment with E2, PPT and DPN. The values of relative expression of target genes were normalized against RPLP0 and displayed in arbitrary units. The "box-and-whisker plot" represents the median value with 50% of all data falling within the box. The whiskers extend to the 5th and 95th percentiles. An asterisk indicates a significant (p < 0.05) difference compared to the OvxC group.