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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Bacterial lipopolysaccharide induces apoptosis in the trout ovary

Figure 4

Effects of lipopolysaccharide (LPS) on oocyte maturation in brook trout. A. Effects of in vivo LPS administration on brook trout oocyte maturation. Preovulatory trout follicles from saline- and LPS-treated fish were incubated for 48 h at 12°C in the presence of 17α,20β-dihydroxy-4-pregnen-3-one (maturation-inducing steroid or MIS;100 ng/ml). At the termination of the incubation period, follicles were scored for the germinal vesicle breakdown (GVBD). Each bar represents the mean ± SEM of five fish for each treatment, with each assayed in triplicate. B. Dose-response of LPS treatment in vitro on brook trout oocyte maturation. Normal trout preovulatory ovarian follicles were incubated with MIS (100 ng/ml) and in the absence or presence of different concentrations of LPS (0–50 μg/ml) for 48 hours and scored for GVBD. The results show the mean ± SEM from three separate experiments, with each assayed in triplicate. C. Time course of LPS treatment in vitro on brook trout oocyte maturation. Normal trout preovulatory ovarian follicles were preincubated in the absence or presence of LPS (25 μg/ml) for different amounts of time (0–48 hours) and subsequently incubated in the presence of MIS (100 ng/ml), as indicated above, and assayed for GVBD. The results show the mean ± SEM from three separate experiments, with each assayed in triplicate. In all graphs, the results are expressed as percentage of total ovarian follicles at the peripheral GV stage that underwent GVBD. Statistically significant (p ≤ 0.05) differences among groups are indicated by different letters.

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