Steroidogenic output of brook trout ovarian follicles treated withsaline and lipopolysaccharide (LPS) in vivo. Preovulatory trout follicles from saline- and LPS-treated fish were incubated for 18 h at 12°C in the absence or presence of salmon LH (sLH; 25 ng/ml). At the termination of the incubation period, testosterone (A) and 17α-hydroxyprogesterone (B) were measured in the medium. Each bar represents the mean ± SEM of five fish for each treatment, with each assayed in triplicate. The results are expressed as percent change with respect to the saline-injected control (no sLH) group which has been set at 100%. Statistically significant (p ≤ 0.05) differences among groups are indicated by different letters.