Alcohol influenced healing and migration rate of cells differently in mono (A and B) and multilayers (C and D). A. Mean ± standard deviations of wound areas (%) in monolayer cells. Alcohol at high concentration significantly reduced wound areas in monolayer of cells by 6 and 12 h of incubation. Differences in areas between low and high alcohol concentration treated wounds were not significantly different. B. Mean ± standard deviations of % migration rates (μm2/h) of cells in monolayer. Alcohol at high and low concentrations increased migration rate of cells during 3 to 12 h of incubation. Later during 6 to 12 h of treatments, migration rates were increased significantly by only high concentrations of alcohol. C. Mean ± standard deviations of wound areas (%) in multilayer cells. Wounds treated with low concentration of alcohol were larger than the controls and those treated with high concentration of alcohol by 3 h of incubation and this difference was at its maximum by 12 h of incubation. D. Mean ± standard deviations of % migration rates (μm2/h) of cells in multilayers. In multilayer wounds, alcohol at low concentration inhibited migration rate of cells until 12 h. Later (12 to 24 h) the migration rate of cells treated with low concentrations of alcohol was higher than control and those treated with high concentration of alcohol. Symbols Monolayer wounds (○), Multilayer wounds (●), Control (-) or (□), High drug concentration (--) or (■), Low drug concentration (...) or (). * p < 0.05.