Figure 3

Thalidomide reduced healing and migration rate of cells in mono (A & B) and multilayers (C & D). A. Mean ± standard deviations of wound areas (%) in monolayer cells. Wounds exposed to low concentration of thalidomide were significantly larger than controls by 3 h of incubation and this difference increased to maximum by 24 h of incubation. B. Mean ± standard deviations of % migration rates (μm²/h) of cells in monolayer. Migration rates of cells were significantly reduced by treatments with low concentration of thalidomide as early as 3 h of incubation. Later, during 6 to 12 h, the migration rate was reduced by low or high concentrations of thalidomide. At later phase of treatments (24 to 48 h), both high and low concentrations thalidomide increased the migration rate. C. Mean ± standard deviations of wound areas (%) in multilayer cells. Wounds exposed to low or higher concentration of thalidomide were significantly larger than controls (*) by 12 h of treatments. D. Mean ± standard deviations of % migration rates (μm²/h) of cells in multilayer. Migration rates during 3 to 6 h were significantly lower in wounds treated with high concentrations of thalidomide. Later, during 6 to 12 h, both low and high concentrations of thalidomide reduced the migration rate of cells. Symbols Monolayer wounds (○), Multilayer wounds (●), Control (-) or (□) High drug concentration (--) or (■), Low drug concentration (......) or (). * p < 0.05.