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Table 5 Cumulus expansion of COCs incubated in the 10 mM AGT-containing medium supplemented with various concentration of E2

From: Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle

  

Cumulus expansion (%)

Concentration of E2 (ng/ml)

Number of Oocytes examined

0

+1

+2

+3

Control

100

0a

16.2 ± 4.7

25.2 ± 5.0c

58.6 ± 4.3e

0

95

89.8 ± 4.1b

10.2 ± 4.1

0d

0f

100

90

88.4 ± 3.9b

11.6 ± 3.9

0d

0f

500

100

85.9 ± 4.0b

14.1 ± 4.0

0d

0f

1000

95

91.7 ± 0.3b

8.3 ± 0.3

0d

0f

  1. a–b, c–d, e–fColumns with no common superscripts were significantly different (P < 0.05). Values are mean ± SEM of three different replicates. Control: COCs were incubated in medium without addition of 10 mM AGT and E2; AGT: aminoglutethimide; E2: estradiol-17beta.