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Table 5 Cumulus expansion of COCs incubated in the 10 mM AGT-containing medium supplemented with various concentration of E2

From: Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle

   Cumulus expansion (%)
Concentration of E2 (ng/ml) Number of Oocytes examined 0 +1 +2 +3
Control 100 0a 16.2 ± 4.7 25.2 ± 5.0c 58.6 ± 4.3e
0 95 89.8 ± 4.1b 10.2 ± 4.1 0d 0f
100 90 88.4 ± 3.9b 11.6 ± 3.9 0d 0f
500 100 85.9 ± 4.0b 14.1 ± 4.0 0d 0f
1000 95 91.7 ± 0.3b 8.3 ± 0.3 0d 0f
  1. a–b, c–d, e–fColumns with no common superscripts were significantly different (P < 0.05). Values are mean ± SEM of three different replicates. Control: COCs were incubated in medium without addition of 10 mM AGT and E2; AGT: aminoglutethimide; E2: estradiol-17beta.