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Table 4 Cumulus expansion of COCs incubated in the 10 mM AGT-containing medium supplemented with various concentrations of P4

From: Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle

   Cumulus expansion (%)
Concentration of P4 (ng/ml) Number of Oocytes examined 0 +1 +2 +3
Control 100 0a 19.2 ± 6.0 20.1 ± 4.9c 60.7 ± 7.7e
0 110 84.9 ± 5.5b 15.1 ± 5.5 0d 0f
100 105 90.2 ± 3.5b 9.8 ± 3.5 0d 0f
500 115 87.5 ± 1.2b 12.5 ± 1.2 0d 0f
1000 145 89.5 ± 1.3b 10.5 ± 1.3 0d 0f
  1. a-b, c–d, e–fColumns with no common superscripts were significantly different (P < 0.05). Values are mean ± SEM of three different replicates. Control: COCs were incubated in medium without addition of 10 mM AGT and P4; AGT: aminoglutethimide; P4: progesterone.