Table 1 Primer used for the real-time PCR study. For each target gene, full and abbreviated names, GenBank accession number of the corresponding rainbow trout sequence and primer sequences are shown. The clone # is consistent with clone numbering in Figure 1 and Tables 2–4.
Target gene | Abbreviated name | GenBank # | Clone # | Forward sequence | Reverse sequence |
|---|---|---|---|---|---|
ovarian aromatase | cyp19a1 | BX083177 | 196 198 | CTCTCCTCTCATACCTCAGGTT | AGAGGAACTGCTGAGTATGAAT |
vitamin K dependent protein S precursor | protein S | BX320624 | 199 200 | ACATGTGGGGGATGTTCATT | GAGGCCATGTTACGGTTTTG |
Cytidine monophosphate-N-acetylneuraminic acid hydroxylase | cmah | BX878414 | 212 | GGAGGCCTGTTCATCAAAGA | CCTGTGTGAAGCTGTCAGGA |
coagulation factor V | cf5 | BX879767 | 235 | AGGGACACACACACACATCC | GAGTTACTGCACGCACCTGA |
pendrin or solute carrier family 26 | slc26 | BX873066 | 236 | CATGCATGGATTCATGGAATAA | TGGATTGGGTGACATCAACA |
vasotocin | avt | CA375992 | 238 | GAGGCTGGAGGAAGAGTGTG | TTCTGTTTGCTGGGTGACTG |
angiotensin-converting enzyme 2 | ace2 | BX867294 | 245 | AACAACAGGAAGCCAGGATG | CGTTCCACATGTATGCCTTG |
CXC chemokine L14 | cxcl14 | BX868653 | 250 | CAAAGGGAACGAGTGAGAGAA | GCCTGATGGCCAACTTAAAC |
A Disintegrin And Metalloproteinases 22 | adam22 | CA363158 | 258 | CCCGACTAGGAGAGTTGCAG | ATCATCACATGACCCCCACT |
serine protease 23 | sp23 | BX087643 | 296 | ACTGCCGAGAAGGATGAAGA | CCTCAGCAAGGGAAGTGAAG |
aquaporin 4 | aqp4 | BX885214 | 305 306 | TGTCATTACCAGCCAACTGC | TGAGACAGCCCTCCAGAAGT |
elongation factor 1 alpha | ef1 α | AF498320 |  | AGCGCAATCAGCCTGAGAGGTA | GCTGGACAAGCTGAAGGCTGAG |
18S ribosomal RNA | 18S | AF308535 | Â | CGGAGGTTCGAAGACGATCA | TCGCTAGTTGGCATCGTTTAT |