Regulation of ACh production in GCs by FSH. A: Immunostaining of ChAT in GFSHR-17 cells and B: lack of staining in control cells incubated with mouse serum instead of the specific antibody. Bar = 10 μm. C: Rat GFSHR-17 cells incubated for 24 h with 0.5 mIU/ml of porcine FSH respond with a significant increase in ACh production. Results shown represent means and SEM of 8 samples per group. Results of untreated cells were set to 1 (t-test; p < 0.05).