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Table 3 RNA expression. Results of RNA expression for the 37 genes. Nine genes are analysed by both Northern and comparative RT-PCR. For comparative RT-PCR the regulation was determined using cDNA dilutions from control cells and FSH treated cells as in Figure 1 and 2. In the Table, C = FSH corresponds to non regulated genes by FSH, FSH>C corresponds to up-regulated genes and C>FSH corresponds to down-regulated genes. NI corresponds to Non Interpretable, 0 corresponds to no signal. * these genes gave no signals during macro-array screening and thus were not controlled by Northern.

From: Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization

clones

putative identification

Northern

RT-PCR result

primers

Size of PCR product (pb)

H2-146

ACTA2

C>FSH

   

H2-96

ACTG1

C>FSH

   

H1-2

AKR1C3

FSH>C

 

U: GATCATCTCCAGCTGCTTGT

L: AGGAGCTTCTGCCTAAGGAT

123

H2-222

ANXA5

C>FSH

   

H1-98

ATP6

NI

C = FSH

U: TACGGCTAGGGCTACTGG

L: TTCCGCCATAAAACCAAAAC

126

H1-138

CAPNS1

C = FSH

C = FSH

U: ACGGGGTGAGTCTAATGC

L: CCAGTGAAGTGCCCAGAA

348

H1-357

CCT3

FSH>C

   

H1-124

CLU

C = FSH

C = FSH

U: ACCCCAGCGTG CCTCT

L: TTCCTCCGCCACAGTCTC

122

H1-61

Col4A5

FSH>C

FSH>C

U: CATAAAAAGCACAGGGAAAAG

L: TTTCTCTTCTGCTGTATTCG

172

H2-108

COX II

FSH>C

   

H1-280

CYP11A1

NI

FSH>C

U: CAAGTCATTCACGAGGTATC

L: AGGAGATGCTGCGGGAG

161

H1-185

GPX3

FSH>C

   

H2-105

HP1BP3

*

FSH>C

U: CTCAAGTCCATTTCCCAGC

L: TCTGGCTCCTTGCGTGCT

114

H1-1

HSD3B1

FSH>C

   

H2-265

INPP1

C>FSH

   

H2-175

MATR3

*

C>FSH

U: GTTTTTGTTTTATCAGAATGG

L: CTTATCTTTCTTAGTGGTGC

91

H1-100

NACA

C = FSH

C = FSH

U: AAG AGG AGA GTG AAG AGG A

L: TTGAAACACCAAAAAAAGGGT

197

H1-242

NADH1

0

C = FSH

U: TTGGTGAATAGTTTTAGGGC

L: CGAAAGGACAAGAGAAATGG

360

H1-33

PDX2

NI

C = FSH

U: TGACCCAGGAAAGCCAGA

L: CTATGTCGCTCCAGGAAA

129

H1-14

porcine endogenous retrovirus perv-MSL

0

FSH>C

U: AAAACAGCAAAAAACCTAACC

L: GCCCGTCTAACAAGAAAGC

180

H1-73

RP515A

C = FSH

   

H1-129

SCAP

FSH>C

FSH>C

U: CCAGCCCAGATCCAGTG

L: TAAACTACGGGGACCTGT

250

H1-90

SCARB1

NI

FSH>C

U: CAAGAAGCAAGACTGTAGG

L: TGGCAACGGGAGGTGAG

160

H1-62

SLC25A6

NI

C = FSH

U: GTTCTCTTTTGCACAGCCG

L: TTTTTTTGTGTCCTGATTTTATT

86

H2-44

THBS1

C>FSH

 

U: CTTTTCGTCTCCCTGGAAAT

L: TGAAACTGATGGGCAAATCT

529

H1-94

TNC

0

C>FSH

U: GCTCCGTGGTGGACCTG

L: CCCTCCACCTTCAGCTTG

104

H1-305

VG5Q

0

C = FSH

U: CCAGCATAAGCATCATTTTC

L: AACAGAGAACCACCCTCCT

174

H2-37

VIM

0

C = FSH

U: GCTCAAGGGGACCAACGA

L: TGGAAGAGGCAGAGAAATC

253

H2-95

New

0

C>FSH

U: AGTCACCAATCTTATCTCCA

L: ATGAAACAATAGTCCAGGAAG

119

H1-202

unknown

C = FSH

C = FSH

U: ATCCTCAGTCAAAAGTGGCA

L: CAATCAGAATCACTGTGCGT

154

H2-288

unknown

*

C = FSH

U: AAGGTGTGTTGATGTATTTTA

L: TACAGGAAGGGAAGCATC

77

H1-294

unknown

FSH>C

FSH>C

U: CTTAGTTTCAGACTGGAGTT

L: TTTAGCGGCTTGTTCACTCA

104

H2-92

unknown

*

FSH>C

U: ATGCCAACATCATCACCTCT

L: CCCTCTAAACTGGGATCCAT

351

H2-170

unknown

FSH>C

FSH>C

U: AGACTTGTAGGTAGAGGTGA

L: TAGGGTTTTTGGGGTATTTTT

99

H1-180

unknown

FSH>C

FSH>C

U: AAA TTG TAG GTA TAT GTG TCA

L: TAGAGAATAAAATGGTCAGTAA

123

H1-246

unknown

FSH>C

 

U: CAATTCACCATCATCCACAA

L: CCCAGCATTTCATACTGACC

284

H1-276

unknown

FSH>C

 

U: ACGATTCCTCTACTGAAAGCTG

L: TGTTTTTGGCAAACAGCAG

247