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Table 1 Differential screening by macro-arrays. This table summarises the results of differential screening by SSH and the results of sequence analysis: Forward subtraction corresponds to H1 SSH and up-regulated genes by FSH (FSH/C). Reverse one corresponds to H2 SSH and down-regulated genes by FSH (C/FSH). Random selection corresponds to clones that provided no differential signals or no signal at all in both forward and reverse subtraction.

From: Identification of differential gene expression in in vitro FSH treated pig granulosa cells using suppression subtractive hybridization

 

Forward (FSH/C)

reverse (C/FSH)

random selection

total

number of clones

226

275

 

501

number of selected clones after screening

28 (12.4%)

19 (6.9%)

29

76

different clones

20

15

29

64

different genes

18

12

27

55