Figure 3

Inhibitory effect of the dynamin K44A and β-arrestin (319–418) contructs on the FSH-R internalization. A: FSH-R internalization in HEK 293 cells after 8 minutes ¹²⁵I-FSH stimulation. Cells were transfected in triplicates with a plasmid encoding the FSH-R and with 600 ng or 2 μg of empty plasmid or plasmid expressing dynamin K44A or β-arrestin (319–418). B: Kinetics of FSH-R internalization in the presence (triangles) or absence (diamonds) of overexpressed (319–418) construct. C: Kinetics of FSH-R internalization in the presence (triangles) or absence (diamonds) of overexpressed K44A dynamin.