Figure 1

FSH stimulates ERK phosphorylation in HEK 293 cells expressing the rat R-FSH. A: Quantification of the P-ERK/ERK ratio (n = 3 independent experiments) in response to increasing doses of FSH for 6 min. B: Representative autoradiograph of dose-dependent FSH-induced ERK phosphorylation. C: Quantification of P-ERK (n ≥ 3 experiments) in response to FSH stimulation from 2 to 15 min. D. Representative autoradiograph showing the time-course of FSH-induced ERK phosphorylation in cells expressing the FSH-R or transfected with an empty plasmid, as indicated. In 1A and 1C, results are expressed as means + S.E.M. of fold stimulation over basal level. Shared superscripts indicate no significant difference, while different superscripts indicate significant differences at the P > 0.05 level.