Table 3 Variables measured in response to different hormone treatments including toad length, weight, the number and percentage of spawning toads, the number of oocytes, percent fertilization, number of neurulas, percent neurulation, and total number of neurulas. Treatment 7* was administered two doses of progesterone 12 hrs apart. Data in the table are expressedas Means ± SE and ranges of spawning toads.
From: Progesterone improves the number and quality of hormone induced Fowler toad (Bufo fowleri) oocytes
Treatment | Treatment | Length (mm) | Weight (g) | No. and percent of toads spawning | No. oocytes per. spawning toad | Total oocytes | Percent fertilization | Neurulas | Percent neurulation | Total neurulas |
|---|---|---|---|---|---|---|---|---|---|---|
1 | 500 IU hCG + 4 μg LHRHa | 63.5 ± 6.7 | 48.0 ± 18.0 | (2/7) 29% | 2283 ± 269 | 4566 | 0% | 0 | 0% | 0 |
2 | 20 μg LHRHa | 69.4 ± 8.5 | 50.2 ± 17.9 | (0/7) 0% | NA | 0 | NA | NA | NA | NA |
3 | 20 μg LHRHa + 0.25 mg Pimozide™ + 5 mg progesterone | 70.0 ± 8.0 | 48.8 ± 11.4 | (4/7) 58% | 2486 ± 825 (350–4140) | 9944 | 35 ± 11% (0–69%) | 546 ± 60 (0–1131) | 22 ± 12% (0–53%) | 2184 |
4 | 20 μg LHRHa + 0.25 mg Pimozide™ | 65.9 ± 4.5 | 43.5 ± 5.7 | (1/7) 14% | 627 | 627 | 34% | 157 | 25% | 157 |
5 | 60 μg LHRHa + 5 mg progesterone | 67.1 ± 4.7 | 46.0 ± 6.1 | (5/7) 71% | 2004 ± 196 (1489–2625) | 10020 | 73 ± 5% (57–89%) | 378 ± 106 (0–1081) | 19 ± 11% (0–60%) | 1890 |
6 | 60 μg LHRHa + 5 mg progesterone + 0.25 mg Pimozide™ + 500 IU hCG | 65.1 ± 5.0 | 43.2 ± 7.2 | (6/7) 85% | 1078 ± 502 (80–3470) | 6468 | 20 ± 8% (0–47%) | 129 ± 31 (0–178) | 12 ± 5% (0–34%) | 774 |
7* | 5 mg progesterone then after 12 hrs another 5 mg progesterone | 64.0 ± 2.8 | 40.0 ± 5.3 | (0/5) 0% | NA | 0 | NA | NA | NA | NA |