Table 1 Sequences, annealing temperature and size for the gene-specific primers
Genes | Primers | Annealing temp. | Size bp |
|---|---|---|---|
Syndecan 3 | 5'-ACC CCA ACT CCA GAG ACC TT-3' 5'-TTA CTC CAC CAT CGA CAC CC-3' | 60°C | 159 bp |
Connexin 43 (Cx 43) | 5'-GGC GTT AAG GAT CGG GTT AA-3' 5'-CGA CGA CCT GTA CTT AAT GTC GGT-3' | 60°C | 363 bp |
28S | 5'-TGCAGATCTTGGTGGTAGTAGC-3' 3'-AGAGCCAATCCTTATCCCGAAGTT-5' | 58°C | 552 bp |