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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Evidence of a local negative role for cocaine and amphetamine regulated transcript (CART), inhibins and low molecular weight insulin like growth factor binding proteins in regulation of granulosa cell estradiol production during follicular waves in cattle

Figure 3

Expression of CART mRNA and follicular fluid concentrations of CART peptide in healthy estrogen-active dominant follicles (DF) and atretic estrogen-inactive subordinate follicles (SF) collected at random stages of the first follicular wave. A) Intrafollicular concentrations of estradiol (E) in estrogen-active DF and estrogen-inactive SF collected at random stages of the first follicular wave (n = 5 each; least square means +/- SEM; * P < 0.05 versus SF). B) Relative abundance of CART mRNA in estrogen-active DF and estrogen-inactive SF collected at random stages of the first follicular wave (n = 5 each). Expression of CART mRNA was normalized relative to concentrations of β-actin mRNA and expressed as ratio of femtograms CART mRNA to picograms β-actin mRNA (least square means +/- SEM). Expression of CART mRNA was higher in estrogen-inactive SF relative to healthy estrogen-active DF (* P < 0.05). C) Intrafollicular concentrations of CART peptide in estrogen-active DF and estrogen-inactive SF collected at random stages of the first follicular wave (n = 4 each; least square means +/- SEM). Concentrations of CART peptide were also greater in follicular fluid of estrogen-inactive SF relative to estrogen-active DF (* P < 0.05). Reproduced from Kobayashi et al., 2004 [59].

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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