Dose-response effect of purified bovine anti-bovine inhibin antibodies on bovine granulosa cell estradiol production. Estrogen-inactive dominant nonovulatory follicles were obtained from a local abattoir. Granulosa cells from each follicle were pooled and treated for 18 hours with different doses of purified bovine anti-bovine inhibin antibodies (purified anti-bINH Ab) or partially purified bovine anti-bovine inhibin antibodies (anti-bINH Ab). Bars represent the mean ± SEM values for triplicate determinations of concentrations of estradiol in media after culture of a single pool of granulosa cells isolated from two follicles (n = 2 cows). Different letters above bars indicate significant (P < 0.05) difference in means. Reproduced from Jimenez-Krassel et al., 2003 .