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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Evidence of a local negative role for cocaine and amphetamine regulated transcript (CART), inhibins and low molecular weight insulin like growth factor binding proteins in regulation of granulosa cell estradiol production during follicular waves in cattle

Figure 1

Dose-response effect of purified bovine anti-bovine inhibin antibodies on bovine granulosa cell estradiol production. Estrogen-inactive dominant nonovulatory follicles were obtained from a local abattoir. Granulosa cells from each follicle were pooled and treated for 18 hours with different doses of purified bovine anti-bovine inhibin antibodies (purified anti-bINH Ab) or partially purified bovine anti-bovine inhibin antibodies (anti-bINH Ab). Bars represent the mean ± SEM values for triplicate determinations of concentrations of estradiol in media after culture of a single pool of granulosa cells isolated from two follicles (n = 2 cows). Different letters above bars indicate significant (P < 0.05) difference in means. Reproduced from Jimenez-Krassel et al., 2003 [58].

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