Figure 3
From: Regulation of ovulation rate in mammals: contribution of sheep genetic models
3H-thymidine incorporation into sheep granulosa cells. Granulosa cells from small antral follicles of 1–3 mm in diameter were incubated with 3H-thymidine for 2 h after 48 h of culture in the presence of 3% fetal ovine serum with or without recombinant human BMP-2 (100 ng/ml), BMP-4 (50 ng/ml), BMP-6 (100ng/ml), BMP-15 (200 ng/ml) or rat GDF-9 (1000 ng/ml). Results represent the labeling index (percentage of 3H-thymidine-labelled cells). Data are expressed as percentages (mean ± SEM) of the labeling index of cells cultured in control condition. Asterisk indicates significant difference (p < 0.05) compared with control.