Figure 4

Protein expression using a monoclonal antibody (ERα-S) raised against the ERα steroid binding domain. 100 μg of protein was loaded. [A] Representative Western blot for ERα and actin (which was used as an internal standard for normalizing the data); the calculated size of the band is indicated on the left-hand side of the gel. [B] Ontogenic profile: ERα protein was expressed relative to actin. Results shown are mean ± S.E.M. from four different dams; no significant change in protein expression was detected.