Figure 6
MCF7 cells express functional TLR3. Total RNA (100 ng) was used to generate cDNA for measurement of expression of TLR3 in MCF7 cells (Lane 1) and RL95-2 cells (Lane 2) (A). The housekeeping gene, GUS, was also included (A). MCF7 cells were plated at 0.2 × 106 cells/well/ml and stimulated with vehicle, Poly I:C (10 μg/ml), Poly dI:dC (1 μg/ml), or LPS (100 ng/ml). A total of 100 μl cell-free supernatant was used to measure IL-6, IL-8, and IP-10 by ELISA (B). Experiments were performed in triplicate and repeated three times. Data shown is representative of three experiments. Error bars indicate standard deviation of three samples per treatment condition. Statistical significance (*) was determined by one-way ANOVA and the Tukey post-hoc test (P < 0.05).