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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: 17beta-estradiol suppresses TLR3-induced cytokine and chemokine production in endometrial epithelial cells

Figure 4

Effects of E 2 and P on endometrial epithelial cells expressing or not expressing hormone receptors. KLE (A) and HEC-1-A (B) cells were plated at 0.2 × 106 cells/well/ml and maintained in CD-F12 for 48 hours prior to experiments. Media was replaced with media containing either 10-8 M E2 or 10-7 M P. Cells remained in hormone-containing media for a total of 66 hours. 18 hours prior to harvest of supernatants, cells were stimulated with the vehicle, Poly I:C (10 μg/ml), or PMA/I (10 ng/ml and 500 ng/ml). ELISA was performed with 100 μl of cell-free supernatant. Data representative of three experiments are shown. Error bars indicate standard deviation of three samples. Statistical significance (*) was determined by one-way ANOVA and the Tukey post-hoc test (P < 0.05).

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