Figure 3
Effects of E 2 and P on TLR3 mRNA and protein expression. cDNA synthesized using 100 ng of RNA from RL95-2 cells treated with 10-8 M E2 or 10-7 M P for 66 hours was used to detect expression levels of TLR3 mRNA by real-time RT-PCR. Data are shown as average cycle threshold ± SD from samples in triplicate for TLR3 and HPRT (A). Average TLR3 quantities (RQ values) ± SD from samples in triplicate are shown by using the housekeeping gene, HPRT, to normalize samples (B). RL95-2 cells were harvested after 72 hours of treatment with 10-8 M E2 or 10-7 M P and labeled with PEαTLR3.7 mAb in order to detect intracellular TLR3 protein by FACS analysis. PE-conjugated mIgG1 was used as an isotype control (C). Experiments were repeated three times. Data representative of three experiments are shown.