Figure 1

Endometrial epithelial cells express ERα mRNA and protein. (A) cDNA was synthesized from 100 ng of RNA isolated and subjected to quantitative Real Time RT-PCR using primer/probe sets for HPRT and ERα. Delta CT was calculated as follows: ERα CT – HPRT CT. ERα quantities ± SD from samples in triplicate are shown, with cell lines expressing the highest levels of ERα mRNA demonstrating the lowest Delta CT value. The Delta CT value representative of zero expression of ERα is shown (black bar). Statistical analysis was determined using ANOVA (P < 0.05). One asterisk (*) represents statistical significance as compared to bars with no asterisk. Two asterisks (**) represents statistical significance as compared to (*) and bars with no asterisk. (B) cDNA was synthesized from 100 ng of RNA isolated and subjected to quantitative Real Time RT-PCR using primer/probe sets for HPRT and ERα. Average ERα quantities (RQ values) ± SD from samples in triplicate are shown by using the housekeeping gene, HPRT, to normalize samples. (C) Protein lysates from endometrial and breast epithelial cell lines were collected and 10 μg of protein was analyzed for presence of ERα protein (67 kD) by Western Blot. Samples shown are as follows: (1) RL95-2 cells positive for ERα mRNA by endpoint RT-PCR, (2) RL95-2 cells negative for ERα mRNA by endpoint RT-PCR, (3) and (4) MCF7 positive control.