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Figure 6 | Reproductive Biology and Endocrinology

Figure 6

From: Differential regulation of follicle stimulating hormone by activin A and TGFB1 in murine gonadotropes

Figure 6

Purified murine gonadotropes express Tgfbr2 at low levels. Purified gonadotropes and gonadotrope-depleted pituitary cells were plated in triplicate in 96 well culture plates. After 48 hrs, total RNA was isolated and real-time RT-PCR was performed to examine Tgfbr1 and Tgfbr2 mRNA expression. Normalized threshold cycle (Ct) values were averaged and used to compare receptor expression in the different cell types and different age groups using the 2-ΔΔCt method for quantitation. Data are presented as mean (+ SD) (A) Tgfbr1 or (B) Tgfbr2 mRNA levels relative to those in young murine gonadotropes (set to 100%). Data from young and old animals were from 3 or 2 independent experiments, respectively. Bars with different letters differed significantly. When averaged across age-groups, Tgfbr2 levels were higher in gonadotrope-depleted cells than in gonadotropes. Note the different scales of the y-axes in (A) and (B).

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