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Figure 1 | Reproductive Biology and Endocrinology

Figure 1

From: Differential regulation of follicle stimulating hormone by activin A and TGFB1 in murine gonadotropes

Figure 1

TGFB1 fails to stimulate Fshb transcription or Smad2/3 phosphorylation in LβT2 cells. A) LβT2 cells were seeded in 6-well plates and transfected with the murine -1990/+1 mFshb-luc reporter. Following transfection, cells were treated with the indicated concentrations of activin A (closed circles) or TGFB1 for approximately 24 hours. Lysates were subjected to luciferase assays. Data points reflect mean (+/- SEM) fold-change in luciferase activity from the control condition (0 nM) in two experiments performed in triplicate (n = 6). Points with different letters differed significantly. B) LβT2 cells seeded in 6-well plates were treated with vehicle (control), 1.2 nM activin A or TGFB1 for 1 hour. Immunoblots (IB) of whole cell lysates were probed with rabbit anti-phospho-Smad2 (top), anti-phospho-Smad3 (middle), or anti-Smad2/3 (bottom) antibodies. Treatments were performed in duplicate. Numbers at the left are molecular weight standards in kDa.

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