Figure 3From: Induction and inhibition of oocyte maturation by EDCs in zebrafishInduction of maturation by tamoxifens. (A) De novo synthesis of cyclin B. Extracts were prepared from twenty oocytes after incubation with EtOH, 17,20β-DHP, DES, TAM or PCP with 17,20β-DHP. Extracts of each treatment were electrophoresed under denaturing conditions (10.0% gel) and immunostained with anti-goldfish cyclin B polyclonal antibody after electroblotting. An arrow indicates the 48 kDa band of cyclin B. (B) Time-course change of germinal vesicle breakdown induced by EDCs. Oocyte maturation induced by 0.01% EtOH (○), 0.1 μM 17,20β-DHP (●), 1 μM DES (Δ) 100 μM TAM (×) or 50 μM 4-OHT (+). Each panel indicates separate experiments using three different females. (C) Inhibition of oocyte maturation by anti-mPRα antibody. Oocytes were incubated with 100 μg/ml of anti-goldfish mPRα polyclonal antibody (Anti) or control IgG (Cont) for 1 hour at 25°C, then oocytes were treated with 10 μM TAM for 3 hrs. %GVBD was calculated by determining the percentage of oocytes that had undergone germinal vesicle breakdown (GVBD) in a group of more than 20 oocytes. Each value is the mean of three separate experiments using ovaries from three separate females. Vertical bars show standard deviation. **indicates significant differences between the %GVBD induced in control- and anti-mPRα antibody-treated oocytes at the P < 0.01 level.Back to article page