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Figure 3 | Reproductive Biology and Endocrinology

Figure 3

From: Induction and inhibition of oocyte maturation by EDCs in zebrafish

Figure 3

Induction of maturation by tamoxifens. (A) De novo synthesis of cyclin B. Extracts were prepared from twenty oocytes after incubation with EtOH, 17,20β-DHP, DES, TAM or PCP with 17,20β-DHP. Extracts of each treatment were electrophoresed under denaturing conditions (10.0% gel) and immunostained with anti-goldfish cyclin B polyclonal antibody after electroblotting. An arrow indicates the 48 kDa band of cyclin B. (B) Time-course change of germinal vesicle breakdown induced by EDCs. Oocyte maturation induced by 0.01% EtOH (), 0.1 μM 17,20β-DHP (), 1 μM DES (Δ) 100 μM TAM (×) or 50 μM 4-OHT (+). Each panel indicates separate experiments using three different females. (C) Inhibition of oocyte maturation by anti-mPRα antibody. Oocytes were incubated with 100 μg/ml of anti-goldfish mPRα polyclonal antibody (Anti) or control IgG (Cont) for 1 hour at 25°C, then oocytes were treated with 10 μM TAM for 3 hrs. %GVBD was calculated by determining the percentage of oocytes that had undergone germinal vesicle breakdown (GVBD) in a group of more than 20 oocytes. Each value is the mean of three separate experiments using ovaries from three separate females. Vertical bars show standard deviation. **indicates significant differences between the %GVBD induced in control- and anti-mPRα antibody-treated oocytes at the P < 0.01 level.

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