Figure 1

Induction and inhibition of oocyte maturation by EDCs. (A) Oocyte maturation-inducing activity of EDCs was examined. Each value is the mean of three separate experiments using ovaries of three different females except for TAM and 4-OHT (n = 6). (B) Effects of EDCs on 17,20β-DHP-induced oocyte maturation were examined. Each compound was added to the medium, then 0.1 μM of 17, 20β-DHP was applied. %GVBD was calculated by determining the percentage of oocytes that had undergone germinal vesicle breakdown (GVBD) among more than 20 oocytes cultured for three hours. Each value is the mean of three separate experiments using ovaries of three different females. Vertical lines indicate standard deviation. A logistic function was used to fit the dose-dependence. Each symbols represents as following: —●—, 17,20β-DHP; —○—, DES; --■--, 17α-Estradiol; —□—, 17β-estradiol; --■--, ethynylestradiol; --□--, resveratorol; —◆—, o,p'-DDT; --◆--, p,p'-DDT; —◇—, butyl benzyl phthalate ; ----◇--, di(2-ethylhexyl)phthalate; —▲—, pentachlorophenol; —▼—, bisphenol A; --▼--, p-nonylphenol; —▽—, octylphenol; --▲--,TAM; —△—, 4-OHT; --▽--, methoxychlor; --△-- methoxychlor (1 hr pre-incubation).