Figure 8
Quantitative real-time RT-PCR analysis of (A) bPRP-VIII and (B) bPRP-IX mRNA in bovine placenta. The total RNA was extracted from cotyledons containing extra-embryonic membrane (EEM), cotyledonary placenta (COT), intercotyledonary fetal membrane (ICOT), caruncular placenta (CAR), and intercaruncular endometrium (ICAR) on Day 27, Day 60, Day 150, and Day 250 of gestation. The expressions of these mRNAs were normalized to the expression of GAPDH measured in the same RNA preparation. Values are means ± SD. Values with different letters are significantly different (P < 0.05).