Figure 1

Representative saturation curve (A) and Scatchard analysis (B). Specific binding of [³H]-17,20β-P to the plasma membrane fraction of Arctic char ovaries using membrane suspensions incubated with [³H]-17,20β-P at concentrations ranging between 1 nM – 20 nM with or without a 100 fold excess non radiolabeled 17,20β-P. Specific binding (▼) was determined by subtracting non-specific binding (■) from total binding (▲). TB: total binding, NSB: nonspecific binding, SB: specific binding. All points are means of triplicate determinations.